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KMID : 0880220230610020233
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Journal of Microbiology
2023 Volume.61 No. 2 p.233 ~ p.243
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Alpha?Hemolysin from Staphylococcus aureus Obstructs Yeast?Hyphae Switching and Diminishes Pathogenicity in Candida albicans
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Xiaoyu Yu
Yinhe Mao
Guangbo Li
Xianwei Wu
Qiankun Xuan
Simin Yang
Xiaoqing Chen
Kim Ji-Eon
Jian Guo
Jinhu Guo
Wenjuan Wu
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Abstract
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The use of antibiotics can disrupt the body’s natural balance and increase the susteptibility of patients towards fungal infections. Candida albicans is a dimorphic opportunistic fungal pathogen with niches similar to those of bacteria. Our aim was to study the interaction between this pathogen and bacteria to facilitate the control of C. albicans infection. Alpha-hemolysin (Hla), a protein secreted from Staphylococcus aureus, causes cell wall damage and impedes the yeast?hyphae transition in C. albicans. Mechanistically, Hla stimulation triggered the formation of reactive oxygen species that damaged the cell wall and mitochondria of C. albicans. The cell cycle was arrested in the G0/G1 phase, CDC42 was downregulated, and Ywp1 was upregulated, disrupting yeast hyphae switching. Subsequently, hyphae development was inhibited. In mouse models, C. albicans pretreated with Hla reduced the C. albicans burden in skin and vaginal mucosal infections, suggesting that S. aureus Hla can inhibit hyphal development and reduce the pathogenicity of candidiasis in vivo.
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KEYWORD
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Candida albicans, Hemolysin, Hyphal development, Cell wall, Mitochondria, Cell cycle, Pathogenicity
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